Product Description:
WO2015118152A1 – Proteomic sample preparation using
Ultrafiltration (Filter-assisted-Sample-Preparation, FASP) (Wisniewski, Resulting peptides can be used in downstream HPLC-based fractionation methods.
Comparative Quantitative Mass Spectrometry Analysis of MHC
May 12, 2010 sample were pooled and prepared for iTRAQ labeling. Two … MHC class II positive fractions from RP-HPLC were pooled for each sample and.
Titan3 and Target2 Chromatography Syringe Filters – Fisher Scientific
particulates. Both Titan3 and Target2 syringe filters provide high-quality filtration solutions. • Sample preparation for HPLC and UHPLC analysis.
DETECTION OF FOOD PROTEINS IN HUMAN SERUM USING
chromatography-tandem mass spectrometry (LC-MS/MS) methods for in vivo detection fractions was a highly effective separation and identification tool.
Comparison of 4-plex to 8-plex iTRAQ Quantitative Measurements of
May 17, 2012 Methods for isobaric tagging of peptides, iTRAQ or TMT, … for fractionation based on isoelectric point and subsequently RP-HPLC in TempoLC …
Plasma protein profiling of Mild Cognitive Impairment and
Jan 17, 2014 To remove potentially iTRAQ incompatible buffer components, a portion of each sample comprising 100 μg of total protein was precipitated for two …
Comprehensive Analysis of the Global Protein Changes That Occur
Eight hundred micrograms of the iTRAQ-labeled mixed peptide samples was separated by high-pH C18 reversed phase high performance liquid chromatography (RP-HPLC)
Analysis of the Escherichia coli extracellular vesicle proteome
Jun 24, 2019 Using 8-plex iTRAQ approaches, we profiled the EV proteome of two … by filtration through 0.22 μm PES syringe filter (Merck Millipore), …
“Isolation of Phosphopeptides by Immobilized Metal Ion Affinity
This protocol describes the fractionation of a complex peptide mixture by cation- Biosystems iTRAQ), with holder and syringe adapter.
Proteomic Analysis of Unbounded Cellular Compartments: Synaptic
Aug 25, 2016 Filter 2 retains cleft-enriched proteins over general cell-surface proteins, on the basis of 114/116 or 115/116 iTRAQ ratio. Filter 3 removes …
Comparative Analysis of Label-Free and 8-Plex iTRAQ Approach for
Sep 2, 2015 Even though pre-fraction of the iTRAQ labeled peptides allowed for a … The solution was filtered using syringe driven filter unit (0.22 μM …
Analytical platforms in vitreoretinal proteomics | Bioanalysis
Dec 11, 2014 SCX is commonly used for peptide fractionation in iTRAQ studies of retinal samples [57,63,107]. In a study upon RGCs, Hollander et al.
Intro to Proteomics 1.0 – College of Biological Sciences |
fractionation of protein and peptide mixtures prior to analysis HPLC or SPE. Proteins. Peptides. Mass Spec. FT-ICR. Mass Spec. Analysis. Analysis.
Preparation of Mitochondria from Ovarian Cancer Tissues – JoVE
Nov 18, 2019 Fractionate the mixed iTRAQ-labeled peptides with SCX chromatography into 60 fractions (one fraction per 1 min), then combine every two …
Sample preparation and fractionation techniques for intact proteins
Nov 2, 2020 Separations are easily performed with a syringe, a pump, a centrifuge, or a chromatography system. 6.2.2 Molecular weight cut-off filters. MWCO …