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iTRAQ proteomic analysis of extracellular matrix remodeling
Dec 01, 2015 · The proteome of the samples was analyzed by 2D-LC MS/MS iTRAQ methodology. The results showed an altered expression of 13 ECM proteins of which 3 (biglycan, periostin, prolargin) were validated by Western blotting and/or SRM analyses. These findings are substantiated by our previous results demonstrating differential ECM protein expression.
Sub-proteomic fractionation, iTRAQ, and OFFGEL-LC-MS/MS
Apr 12, 2010 · In the current, study we combined rat cardiac sub-proteomic fractionation, iTRAQ, and OFFGEL as an approach to quantify protein abundance differences between ischemic and non-ischemic cardiac tissue. We found 22 proteins involved in regulating intracellular Ca 2+ , thin filament length, metabolism, cytoskeleton structure, and transport that changed significantly in abundance.
Minimising iTRAQ ratio compression through understanding LC
Mar 21, 2011 · Here, we also investigate in greater detail the dependency of iTRAQ quantification on the dynamics of online chromatography in low-complexity mixtures (iTRAQ-labelled standards). These findings will allow more efficient strategies to be designed for iTRAQ proteomics, alleviating iTRAQ underestimation and thus facilitating the detection of
Sub-proteomic fractionation, iTRAQ, and OFFGEL-LC-MS/MS
Jun 16, 2010 · Using an in solution based approach with a sub-proteomic fraction enriched in cardiac sarcomeric proteins; we identified protein abundance in ischemic and non-ischemic regions of rat hearts stressed by acute myocardial ischemia by ligating the left-anterior descending coronary artery in vivo for 1h without reperfusion.
fractionation iTRAQ hplc syringe filters-HPLC Filter
May 12, 2010 sample were pooled and prepared for iTRAQ labeling. Two MHC class II positive fractions from RP-HPLC were pooled for each sample and. Titan3 and Target2 Chromatography Syringe Filters – Fisher Scientific. particulates. Both Titan3 and Target2 syringe filters provide high-quality filtration solutions.
Quantitative subproteomic analysis of age-related changes in
Nov 15, 2011 · Our analysis led to identification and quantification of 150 proteins, 8 out of which were differentially expressed between two age groups at a statistically significant level (p<0.05), with folds ranging from 1.2 to 4.1. These proteins involved in peroxisomal β-oxidation, detoxification of xenobiotics and production of ROS.
Optimization of iTRAQ labelling coupled to OFFGEL
Jun 06, 2012 · The present work describes a feasible and novel protocol for in-solution protein digestion in which the filter unit permits protein retention and buffer removal. The data demonstrates an impact of iTRAQ labelling on peptide electrofocusing behaviour in OFFGEL fractionation compared to their native c
SubProteomic fractionation iTRAQ and OFFGELLCMS hplc filter
Partial HPLC chromatograms of the fractionation of iTRAQ labeled. The analysis of plasma samples from HIV-1/HCV mono- and coinfected individuals by quantitative proteomics is an efficient strategy to investigate changes in Sub-Proteomic fractionation, iTRAQ, and OFFGEL-LC-MS – NCBI
Quantitative subproteomic analysis of age-related changes in
Nov 15, 2011 · Our analysis led to identification and quantification of 150 proteins, 8 out of which were differentially expressed between two age groups at a statistically significant level (p<0.05), with folds ranging from 1.2 to 4.1. These proteins involved in peroxisomal β-oxidation, detoxification of xenobiotics and production of ROS.
Sub-proteomic fractionation, iTRAQ, and OFFGEL-LC–MS/MS
Jun 16, 2010 · Peptides that were cleaned up with the strong cation cartridge also had to be loaded onto the Vydac column to remove contaminants prior to OFFGEL fractionation. The HPLC used for the Vydac column was a Dionex U-3000 analytical system equipped with a UV detector (214 nm) run at 1 ml/min with separation solution B 95% ACN, 0.5% TFA run in a step wise gradient from 0.0% B for 10 min, 80% B for 10 min, 80%–100% in 2 min, held at 100% B for 5 min, 0.0% B for 7 min. All peptides were eluted in
Subproteomic analysis of basic proteins in aged skeletal
We, therefore, carried out a comparative subproteomic study of young versus aged rat muscle focusing on potential changes in muscle proteins with an alkaline isoelectric point, using a combination of offgel electrophoresis and two-dimensional (2D) slab gel electrophoresis. Offgel electrophoresis was successfully applied as a prefractionation
KaiUwe Eichhorn SubProteomic fractionation hplc syringe filters
Use syringe filters to extend column lifetime, flow rates & loading volumes when preparing small-volume samples for HPLC and ion chromatography. Learn more. HPLC and Chromatography – Hangzhou Anow Microfiltration Co.,Ltd.
Sub-Proteomic fractionation, iTRAQ, and OFFGEL-LC-MS/MS
Jun 06, 2010 · In the current, study we combined rat cardiac sub-proteomic fractionation, iTRAQ, and OFFGEL as an approach to quantify protein abundance differences between ischemic and non-ischemic cardiac tissue. We found 22 proteins involved in regulating intracellular Ca 2+ , thin filament length, metabolism, cytoskeleton structure, and transport that changed significantly in abundance.
Shotgun proteomics using the iTRAQ isobaric tags | Briefings
May 10, 2006 · The complex mixture of labelled peptides was separated using strong cation-exchange (SCX) fractionation followed by reversed-phase high pressure liquid chromatography (HPLC). The resolved peptides were studied using MALDI TOF/TOF analysis on a 4700 Proteomics Analyzer running v2.0 software.
iTRAQ Proteomics Service – Applied Biomics
iTRAQ is a liquid-based proteomics analysis. It labels the Lysine residues and N terminus of all peptides with isotopes of identical masses, thus allowing quantitative comparisons between 2 (2-plex) to 8 (8-plex) samples. This approach can provide both protein identification and protein ratios in one experiment.